Hi Hazel,
The CV is simply the standard deviation divided by the mean. If you
are sure that your distribution is normal then thats okay but you might
want to consider also a robust CV. Since its a simple calculation its
probably overkill to get special software to calculate just the CV. An
excel spreadsheet is probably the easiest thing. If you wish I can email
you a spreadsheet that should do the trick for you. Also, if you are
doing a lot of data analysis and want a small routine to convert text
files into FCS histogram files there might be something out there or I
can write something quickly for you. Most commercial software packages
should be able to read the histogram format specified in the FCS Standard
(FCS Express can, of course :-) ) and then you can use whichever standard
software you like.
-Dave
Hazel Davey wrote:
> Dear all,
>
> We have a need to calculate the coefficient of variation of some
> flow cytometry data however it is not in FCS format so we can't
> load it into the standard packages.
>
> It is in the form:
>
> channel number1, count
> channel number2, count
> etc
>
> Does anyone know of a software package that can calculate the cv
> of such data for us? All advice hints and tips greatfully received as
> the final year PhD student in question needs cv's for a pile of files
> for her thesis.
>
> On a related note, calculating cv's is very dependent on where you
> place boundaries around the peak, any favourite peak picking
> methods out there?
>
> Cheers,
>
> Haze
> ----------------------------------------------------------
> | Hazel Davey hlr@aber.ac.uk |
> |Sefydliad y Gwyddorau Biolegol*Inst. Biological Sciences|
> |Prifysgol Cymru * University of Wales|
> | ABERYSTWYTH, Ceredigion, CYMRU / WALES SY23 3DD |
> | http://pcfcij.dbs.aber.ac.uk/index.htm |
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:18 EST