Maris, I can't help with the first, but for the second I got a lot out of Video Microscopy by the legendary Shinya Inoue, and Digital Image Processing, by Gregory Baxes. The latter is a skinny thing, but very helpful. For a solid introduction to both subjects I strongly suggest the Woods Hole Microscopy course, probably starting next week (too late). A word of caution: it's intense. When they say "check in by 3pm", that's because lectures start at 3:15, and you'll be in lab till 11pm! Ahhh, good times.... +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Steve G. Hilliard (706) 542-9474 University of Georgia Cell Analysis Facility flowman@uga.edu http://floweb.cb.uga.edu/ On 2 May 2000, Maris Handley wrote: > > Hi Flowers, > > I have two issues I'm hoping you can help me with: > 1. If anyone using a good protocol for 7AAD (for cell cycle, not viability), with FITC > and PE surface staining, could you send me the protocol or point me towards a reference? > 2. Any suggestions on books for microscopy and/or image analysis? > > Thanks! > Maris > > > Maris Handley > Dana-Farber Cancer Institute > Boston, MA > (617)632-3179 > maris_handley@dfci.harvard.edu >
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