After incubation of ovarian carcinoma cell lines with cis-platin, the apopttoic cells detach from the culture dish and can be found flowting in the sulture medium (for example, see: Cisplatin induces apoptosis in a human ovarian carcinoma cell line without concomitant internucleosomal degradation of DNA. M.G. Ormerod, C.F. O'Neill, D. Robertson and K.R. Harrap. Expl. Cell Res.,206, 231-237 (1994); Cis-diamminedichloroplatinum(II) induced cell death through apoptosis in sensitive and resistant human ovarian carcinoma cell lines. M. G. Ormerod, C. F. O'Neill, D. Robertson, L. Kelland and K. R. Harrap. Cancer Chemotherapy and Pharmacology, 37, 463-471, 1996). In my experience, there are few apoptotic cells to be fuond still attached to the culture dish. To count the number of apoptotic cells, having confirmed microscopically that the 'floating' cells are indeed apoptotic, you only need to count the number of detached cells. Michael Ormerod 34 Wray Park Road Reigate RH2 ODE Telephone: +44 (0)1737 241726 PLEASE NOTE NEW FAX & MOBILE NUMBERS FAX: +44 (0)1737 226736 Mobile telephone: 07802 293242 Web site: http://ourworld.compuserve.com/homepages/Michael_Ormerod
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