Dear Ed:: Cross-reactivity between ICAM-1 and its ligands has often been reported in the literature. For instance, human CD11a/CD18 will bind murine ICAM-1, or ICAM-2, for that matter, but not vice-versa. Binding of swine neutrophils to human ICAM-1 on HUVECs is thus not surprising. But this is very different from cross-reactivity at the level of the antibodies. For instance, antibodies to human and mouse don't cross-react. Theoretically, there are several anti-human ICAM-1 antibodies that are available commercially or that have been raised by private investigators that could be used for a sandwich ELISA for measuring sICAM-1. But since swine ICAM-1 has not been cloned yet, (at least to my knowledge), it is difficult to predict if the human kit will work. The best thing to do would be to try the human kit (it uses a polyclonal and a monoclonal, if I remember well), but the incredibly unreasonnable price of these kits makes you think twice before ordering it !!! Good luck ! Yves ****************************** Yves St-Pierre Professor INRS-Institut Armand-Frappier 531 Boul. des Prairies Laval, QC, CANADA H7V 1B7 Phone: 450-686-5354 Fax: 450-686-5501 E-mail: yves.st-pierre@inrs-iaf.uquebec.caEd Cabrera-EJ wrote: > > This is not directly related to cytometry, but perhaps someone in this > conference has some insight into this issue. > I am interested in measuring sICAM in swine plasma. There are kits available > for human sICAM. Does anyone know if there is a good crossreactivity between > human and swine sICAM. I have shown that neutrophils will adhere to HUVEC thru > a mechanism that is inhibited by swine antiCD18, so I assume that there is a > certain degree of crossreactivity since ICAM-1 is the main counterreceptor for > CD18/CD11b. > > Any help will be appreciated. > > Thanks > > Ed Cabrera > Procter and Gamble Pharmaceuticals > Mason, OH
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