-----Original Message----- From: Janene White [mailto:janene.white@imvs.sa.gov.au] Sent: Wednesday, 26 April 2000 16:53 To: cyto-inbox Subject: 7AAD Trial A researcher asks? Can anybody help with a good explanation of staining with 7AAD then using a cell fixative (paraformaldehyde) instead of PBS buffer. So, instead of using the PBS (as in the methodology) we are trialling the use of a cell fixative (post 7AAD staining) which will allow us increased flexability in work practice. It seems to be quite successful (over a period of days the % of live v's dead remains stable) except we are at a loss to explain the mechanics of how the 7AAD viability dye indicates that a percentage of cells are "live" when theorectically they are all "dead" after cell fixation. Janene Vine Division of Human Immunology (Diagnostic) Institute Of Medical and Veterinary Science Frome Road, Adelaide 5000 E-mail : janene.white@imvs.sa.gov.au Phone : (08) 82223476
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