Maciej: CD11a is expressed on all leukocytes, CD11b &c are expressed on monocytes, granulocytes and NK cells. The CD11's are the alpha chains of beta2 integrin family while CD18 represents the beta chain. CD11b is used most often because it is the most densely expressed on the cell surface (i.e. brightest) and has the largest increase upon stimulation. You don't mention any stimulation of your cells in your procedure, we used 60ng/mL (final concentration) in a whole blood assay. (See O'Gorman et al; Annls NY Acad Sci, 1993; 667:427-430). We once had a patient that had decreased CD11b on resting but normal levels upon stimulation. This child had mild disease. Also, are you gating on lymphocytes as your title suggests? You should really be gating on your granulocytes for CD11b. Since CD11b and CD18 represent different chains of the beta2 integrin, it is feasible to have a defect in one and not the other. I don't know what the clinical significance would be. Any comments Dr. O'Gorman?? Joanne Thomas, M.S. Director of Operations TRITECH Field Engineering 2014 Renard Court, Suite I Annapolis, MD 21401 1-800-886-7004 (USA) 1-410-266-1522 410-266-0935 (FAX) thomas@tritechinc.com -----Original Message----- From: Maciej Simm <simmmmer@yahoo.com> To: cyto-inbox Date: Thursday, April 20, 2000 4:55 PM Subject: CD11b expression on Lymphocytes > >Greetings once again, > >We have recently received a blood specimen and were asked to stain >for CD18/11b ( I love it how doctors don't explain the condition of >the patient ahead of time, so we know what to look for ) > >We found, that when compared to our NC's (N=300+ for this "tube" and >growing) this patient had less +/+ lymphoctes (~30% vs. 12%). The >expression of CD18 was not reduced - all of his leukocytes had >97%. > >A question came up: what is the significance of CD18+/CD11b- >lymphocytes? what is the significance of a decrease in the +/+ >population? > >We do not routinely do CD11a or CD11c - are these mutually exclusive? >I.e. it's either a, b or c but never any of them together? > >And finally, is the ligand for CD11b soluble and may have interfered >with our staning? > >This staining was done in whole blood (which I just found is a >no-no). > >I would appreciate if anyone could share any thoughts on this topic. > >Maciej > >__________________________________________________ >Do You Yahoo!? >Send online invitations with Yahoo! Invites. >http://invites.yahoo.com >
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