We worked on this and found the detection of cyclin D1 to be highly technique dependent. You can not viably freeze the cells, exact procedures must be followed and someone having a bad day- a tiny bit off in timing, etc- can't reproduce this test. Matter of fact, only one person in the lab could reproduce this test. Surprisingly, we found that DNA content analysis was as good at detecting mantle cell lymphoma and much more dependable a method. Since the primary differential diagnosis is between CLL and mantle cell and since CLL doesn't cycle and mantle cell does, you can make a diagnosis. I have talked to many who have tried and come to the same conclusion- don't try to use cyclin D1 in the clinical lab. It is a waste of your time. Maryalice >From: "Dorn-Beineke, Alexandra" ><alexandra.dorn-beineke@ikc.ma.uni-heidelberg.de> >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >Subject: Cyclin D1 >Date: Thu, 20 Apr 2000 11:56:34 +0200 >X-PMFLAGS: 34078848 0 1 9869.CNM > > >Dear Collegues, >we are looking for a simple method to stain Cyclin-D1 for identification of >mantle cell lymphoma (translocation t11;14) by flow cytometry, ideally in >whole blood. Current protocols for cytoplasmic detection seem to fail and >also those published are difficult to reproduce and need significant amounts >of blood. As we get less and less volume of blood this becomes a problem. >Thanks for your support. > >Alexandra Dorn-Beineke >Thomas Nebe > >Dr. med. Alexandra Dorn-Beineke >FÄ für Laboratoriumsmedizin >Universitätsklinikum Mannheim >Abt. Hämatologie, Immunologie, Allergologie >Theodor-Kutzer-Ufer 1-3 >D-68167 Mannheim >Tel: +49 621 383-3561 o. 3486 >FAX: +49 621 383-3819 >e-mail: alexandra.dorn-beineke@ikc.ma.uni.heidelberg.de >http://www.ma.uni-heidelberg.de/inst/ikc/ Maryalice Stetler-Stevenson Director Flow Cytometry Unit Laboratory of Pathology, NCI, NIH
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