"Witherspoon, Sam" wrote:
>
> I'm trying to collect DAPI and APC signals on the second (and third)
> laser lines of a FACStar Plus
> The DAPI signal is very bright... should I reflect it and filter the
> APC fluorescence or should I filter the DAPI and reflect the APC?
Hi Sam,
I recently tried to work this combo out on our Elite. Setup was 488
beam displaced from the other two beams [uv and 633] which were set
colinear. An unexpected result was that DAPI fluorescence showed up in
the APC signal. I tried different bandpass and longpass filters for the
APC and ran samples with variuos lasers blocked to verify the souurce of
the signal. My conclusion was that DA{PI simply fluoresces way out into
the red, at least under the experiemental conditions I used.
Regards,
Tom
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Thomas W. Mc Closkey, Ph. D., Director of Flow Cytometry
North Shore University Hospital, New York University School of Medicine
Boas Marks Biomedical Research Center, 350 Community Drive
Manhasset, Long Island, New York 11030
ph: 516-562-4844 [office], 516-562-1135/4641 [lab] fax: 516-562-2866
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This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:17 EST