antigen retrieval might also work on these cells. I lost the original email address of the person who posted it, but here are references for AR: John: Here are a few pertinent references: 1. Shi,S. et al. Antigen retrieval in formalin-fixed parrafin-embedded tissues:....... J.Histchem.Cytochem.39:741-748 (1991). 2. Taylor,CR et al. Strategies for improving the immunohistochemical staining of... Hum.Pathol.25:263-270 (1990). 3. Momose H. et al. Antigen retrieval by microwave irradiation in lead thiocyanate.... Appl.Immunohistochem.1:77-82 (1993). 4.Leong,A.S-Y. & J. Milios An assessment of the efficacy of the microwave antigen-retrieval... Appl.Immunohistochem.1:267-274 (1993). 5. Cuevas,E.C. et al. Microwave antigen retrieval in immunocytochemistry: A study of 80 Abs. J.Clin.Pathol.47:448-452 (1994). 6. Gown,A.M. et al. Microwave-based antigenic unmasking...... Appl.Immunohistchem.1:256-266 (1993). as far as flozen tissue slides I've worked with it involves simmering the specimen in citrate buffer using microwave oven. Not friendly to morphology. ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` | www.cd4cd8.com | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Send online invitations with Yahoo! Invites. http://invites.yahoo.com
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