Dear Collegues, we are looking for a simple method to stain Cyclin-D1 for identification of mantle cell lymphoma (translocation t11;14) by flow cytometry, ideally in whole blood. Current protocols for cytoplasmic detection seem to fail and also those published are difficult to reproduce and need significant amounts of blood. As we get less and less volume of blood this becomes a problem. Thanks for your support. Alexandra Dorn-Beineke Thomas Nebe Dr. med. Alexandra Dorn-Beineke FÄ für Laboratoriumsmedizin Universitätsklinikum Mannheim Abt. Hämatologie, Immunologie, Allergologie Theodor-Kutzer-Ufer 1-3 D-68167 Mannheim Tel: +49 621 383-3561 o. 3486 FAX: +49 621 383-3819 e-mail: alexandra.dorn-beineke@ikc.ma.uni.heidelberg.de http://www.ma.uni-heidelberg.de/inst/ikc/
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