Dear Flow Cytometerist, I would be grateful for your assistance in answering a question that I have been asked by one of our researchers concerning the identification of G0 cells. We have been investigating G0 cells based on Pyronin Y and Hoechst staining and we have been identifying G0 cells as those that have very low Pyronin Y content and that also appear in the G0/G1 Hoechst peak. What my colleague would like to know is .... "How have people confirmed that cells staining with low Pyronin Y and low Hoechst are G0? Has anyone isolated cells and looked for any other markers of G0?" Any comments or references on this matter would be appreciated. Happy Easter, Jeff Barry Paterson Institute for Cancer Research Manchester UK
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:17 EST