> ---------- > From: shawn_jackson > Sent: Wednesday, 12 April 2000 23:49 > To: Cytometry Mailing List > Subject: annexin V dim cells > > > hey flow-ers > > I have a question concerning the occurrence of annexin V-dim cells vs. > bright > cells. I need help with references and/or data experience! > > I am staining peripheral blood from mice infected with vaccinia, and then > staining the CD8 cells with annexin V-fitc. I see a population of annexin+ > cells extending even up past the 2nd log at many time points, but at > certain > times I see very bright cells up at the 3rd log. > > I have been warned that only these very bright cells are truly AV+, cells > with > any intentions whatsoever of doing anything related to a death process, > while > the dimmer cells represent actively proliferating cells only, with no > intentions of dying. and these dimmer cells are likely to return to an AV- > population quickly. > > Could anyone help me with references or personal experince addressing the > status of these AV dim cells in contrast to bright ones? > > thanks! > > shawn jackson > grad student > harvard univ > boston, ma > > Hello Shawn, > I have recently described early apoptotic neutrophils from peripheral > blood as Ann V dim and late apoptotic neutrophils as Ann V bright (Optimal > storage conditions for preserving granulocyte viability as monitored by > Annexin V binding in whole blood. J Immunol Methods 225 (1999) 27-38. > G.Hodge et al). You could try similar techniques with 7-AAD to determine > if the CD8 Annex V dim cells are early apoptotic cells. > Regards Greg Hodge. (hodgeg@mail.wch.sa.go.au). >
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:16 EST