The general estimate would be how much sample is used per hour divided by how much sheath is used. Or assuming 1 microliter per second of sample is used and that each drop has 3.53 times the cube of the diameter of the nozzle, so 10^6 drops equals 3.53 ml for a 100 micron nozzle. (NOT 0.523 ml) If the pressure is only around 12 psi, then the frequency of drops could be say roughly 2.8 10^4 drops per second) For 35 seconds (10^6 over 2.8 X 10^4): 35 microliters sample per 3.53 ml sheath. So the sample is around 1% of the sorted droplet. "A jet vibrated at the optimum frequency for droplet generation (lambda=4.5d) will produce droplets of volume 3.53d^3. If they were spherical, their diameter would be 1.9 times that of the jet." page 103, Flow Cytometry: Instrumentation and Data Analysis. Eds. Van Dilla, MA, Dean PN, Laerum OD, Melamed MR. Academic Press, 1985. >At 05:40 PM 4/5/00 -0500, you wrote: >> >>Hi cytometry fans, >> >> I have a client that wants to know how much of the drop volume will be >>taken up by the sample fluid (I need a general estimate). We will be using >>an ELITE sorter (12 PSI) with 100 micron sort sense tip to sort. To make my >>life easier, has anyone calculated the sample/sheath ratio after sorting at >>a specific sample input pressure? I thought about running a concentrated >>blue dye and sorting under conditions similar to the eventual client sort >>and then measuring the OD in a spec original sample vs. sorted sample. Has >>anyone tried this and was it possible to see any absorbance in the diluted >>sort sample? Looking forward to your replies, >> >>Kristi Harkins --- Dennis Dennis J. Young Mail:<<mailto:djyoung@ucsd.edu>> WWW:<<http://cancer.ucsd.edu/SResources/flow.htm>> Flow Cytometry Core Facility University of California, San Diego Internal Medicine Group, Bldg #4, Rm 126 9500 Gilman Drive La Jolla CA 92093-0671 Telephone:(858) 822-0407
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