sample/sheath ratio after sort

From: Kristi Harkins (kharkins@iastate.edu)
Date: Wed Apr 05 2000 - 17:40:30 EST 

Hi cytometry fans,

  I have a client that wants to know how much of the drop volume will be
taken up by the sample fluid (I need a general estimate).  We will be using
an ELITE sorter (12 PSI) with 100 micron sort sense tip to sort. To make my
life easier, has anyone calculated the sample/sheath ratio after sorting at
a specific sample input pressure?  I thought about running a concentrated
blue dye and sorting under conditions similar to the eventual client sort
and then measuring the OD in a spec original sample vs. sorted sample.  Has
anyone tried this and was it possible to see any absorbance in the diluted
sort sample?  Looking forward to your replies,

Kristi Harkins

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