Good background/summary chapter on measurement of cell growth and viability can be found in Methods in Enzymology, Volume LVIII Cell Culture, edited by William B Jakoby and Ira H Pastan, Academic Press, New York, 1979 References specific to Trypan Blue (1) R Schrek, Am J Cancer 28,389, 1936 - first to suggest that an intact cell membrane was necessary to exclusion of certain dyes (2) JM Hoskins, GG Meynell & FK Saunders, Exp Cell Res 11, 297, 1956 (3) JR Tennant, Transplantation 2, 685, 1964 (4) W Sawicki, J Kieler, and P Briand, Stain Technol, 42, 143, 1967 (5) HJ Phillips in Tissue Culture: Methods and Applications (PF Kruse Jr & MK Patterson Jr, eds) p406, Academic Press, New York , 1973 (6) S Tolnai, Tissue Cult Assoc Man 1, 37, 1975 Electronic/Flow measurement using Trypan Blue (1) WP Drake, PC Ungaro & MR Mardiney, Transplantation 14, 127, 1972 (2) HA Crissman, PF Mullaney & JA Steinkamp, Methods Cell Biol, 9, 179, 1975 april April G. Durett, MSc Research Associate Cell Processing Laboratory-Flow Cytometry Center for Cell & Gene Therapy Baylor College of Medicine > > -----Original Message----- > From: Peter Kierulf [mailto:peter.kierulf@ioks.uio.no] > Sent: Wednesday, March 29, 2000 12:50 AM > To: Cytometry Mailing List > Subject: Reference- TRYPANE BLUE EXCLUSION > > > > Hi there!, > > > We as many others have been using trypane blue staining to account for > number og dead cells. > > We have tried in vain to find a basic reference that gives information as to > why the cells become trypane blue positive when they are dead. > > Can anyone help out?? > > > Thanks > > > > Peter Kierulf,MD PhD. Professor I > > Clinical Chemistry Dep. > > Ulleval University Hospital > > N-0407 OSLO, NORWAY > > > Telephone: +47 22 11 94 86 > > Telefax: +47 22 11 81 89 > > _-----------------------------------Mobil telphone: 922.85111 > > E-mail (RFC Formate): peter.kierulf@ioks.uio.no > > E-mai. (SA Formate): /G=peter/S=kierulf/OU=ioks/O=uio/PRMD=uninett/C=no > > > Could you please post this message, thanks > > > > Peter Kierulf,MD PhD. Professor I > > Clinical Chemistry Dep. > > Ulleval University Hospital > > N-0407 OSLO, NORWAY > > > Telephone: +47 22 11 94 86 > > Telefax: +47 22 11 81 89 > > _-----------------------------------Mobil telphone: 922.85111 > > E-mail (RFC Formate): peter.kierulf@ioks.uio.no > > E-mai. (SA Formate): /G=peter/S=kierulf/OU=ioks/O=uio/PRMD=uninett/C=no > >
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