On Fri, 24 Mar 2000 14:53:27 -0500, Mark Shlomchik wrote: >To all: >I have a user who claims that the FACSVantage is inferior to the >FACSTAR in discriminating size of cells by FSC. I would a priori see >no reason why this should be true. Am I missing something? Have >others seen this? I doubt if there is an intrinsic difference in FSC discrimination between these models, but there are many things that affect the performance of each individual instrument. The most obvious is simply the alignment of each machine! I've seen significant differences on the one sorter, depending on who set it up and how rushed they were. I now align a FACStar Plus almost every day, and I can detect small differences day to day! Other things are the choice of filter(s) in the FSC path and the setting of the obscuration bar, and these will depend on the configuration of each instrument. Try tweaking it, you'll probably be surprised!~) Good Luck, Joseph. -- Joseph Webster Flow Cytometry Facility Centenary Institute
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