Calman Prussin wrote: >I have to address a reviewers question regarding the effect of Ficoll on the >ratio of CD4 to CD8 T cells. Does anyone in Flowland know of published >articles that have addressed this question? The references in Practical Flow Cytometry are: De Paoli P, Villalta D, Battistin S et al: Re: Selective loss of OKT8 lymphocytes on density gradient separation of blood mononuclear cells. J Immunol Meths 61:259, 1983 Ritchie AWS, Gray RA, Micklem HS: Right angle light scatter: A necessary parameter in flow cytofluorimetric analysis of human peripheral blood mononuclear cells. J Immunol Meths 64:109-17 Renzi P, Ginns LC: Analysis of T-cell subsets in normal adults. Comparison of whole blood lysis technique to Ficoll-Hypaque separation by flow cytometry. J Immunol Methods 98:53, 1987 These were in by the 2nd Edition; I didn't look the topic up specifically for the 3rd because most people were doing whole blood analysis by the time it was in preparation. -Howard
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