Mara, I too use Blood Bank Saline and always have (except for sterile sorting) and have never had any problems. I use S/P brand from Allegiance in the USA. Jeanene Jeanene Swiggett, BS MT(ASCP)SH General Surgery Research Cannon Research Center Carolinas Medical Center Charlotte, NC 704-355-7269 jswigget@carolinas.org > -----Original Message----- > From: Fischer, Randy [SMTP:RFischer@therimmune.com] > Sent: Tuesday, March 28, 2000 8:20 AM > To: Cytometry Mailing List > Subject: RE: sheath fluid for Calibur > > > Mara, > > I have been very happy with VWR's blood bank saline for my FACSCalibur. > > Randy Fischer > TherImmune Research Corporation > 9700 Great Seneca Hwy > Rockville, MD 20850 > (240) 453-6256 > RFischer@therimmune.com > > > ---------- > > From: Mara Rocchi > > Reply To: roccm@blackie.mri.sari.ac.uk > > Sent: Monday, March 27, 2000 4:36 AM > > To: Cytometry Mailing List > > Subject: sheath fluid for Calibur > > > > > > Dear Flowers, > > we are in the process to buy a FACSCalibur, and we would like to here > > your > > suggestion abut the kind of sheath fluid we must/can use. We aren't > > planning > > any sorting, maybe some DNA work, lot and lot of phenotyping and > > intracellular staining. > > From the archives looks like that different people use different > > solutions, > > and for different reasons. I have been using sterile saline for > > FACScan and > > FACStar, and D'PBS for FACSort, but being new to the Calibur world, I > > would > > like to be sure that those alternatives to the BD sheath fluid are OK. > > > > Thanks a million in advance > > Mara > > > > Mara S.L. Rocchi > > Moredun Research Institute > > Penicuik EH26 0PZ > > Scotland, UK > > e-mail: roccm@mf.mri.sari.ac.uk > > > >
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