I have an investigator who is new at flow, and who has a question about apoptosis assays. I directed her to the archives, but she is looking for information from someone who has used cells similar to hers. If you can help, please reply to: dmcaloose@ucdavis.edu. Thanks. Carol I was wondering whether anyone has used either Annexin V or TUNEL flow cytometry on VERY adherent epithelial cell lines in human or non-human species. Specifically, I am looking for a reliable method for detecting apoptosis in normal mammary gland epithelial cells from domestic cats. Without the aid of scraping and in the presence of only trypsin, it takes about 7 minutes to get these cells to lift off of a plate. I am concerned that this either form of cell harvesting will cause enough cell membrane damage to cause the assays to be inaccurate. I would appreciated any feedback, and suggestion of a manufacturer and/or a protocol would be extremely helpful. Thanks, D. McAloose --------------------------------------------------------------------------- Carol Oxford _| _| _|_|_|_| _|_|_| Manager, UC Davis Optical Biology Lab _| _| _| _| _| Tupper Hall, rm 3439 _| _| _| _| _| University of California _| _| _| _| _| Davis, California 95616 _| _| _| _| _| (530) 752-7205 _| _| _| _| _| (530) 752-4548 fax _|_|_|_| _|_|_|_| _|_|_| (530) 523-0967 pager cloxford@ucdavis.edu University of California, Davis ---------------------------------------------------------------------------
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