You might want to try nycoprep mixer where you mix the blood and the cells and later collect the supernatant. I did this on mouse blood some time ago using 1ml eppendorf tubes. However, eventually I changed to whole blood measurements for my application. Regards Gerhard -----Original Message----- From: shawn_jackson [SMTP:shawn_jackson@student.hms.harvard.edu] Sent: Friday, March 24, 2000 11:39 PM To: Cytometry Mailing List Subject: ficoll of small volumes hello all- I am searching for the best way to ficoll small volumes of blood (i.e., 100-200 of mouse blood) efficiently during sample prep for flow. presently I am diluting the blood and spinning in 15 ml polypropylene tubes, but the cell layer can be frighteningly thin. can anyone suggest smaller tubes which have worked? our smaller 12x75mm tubes are awfully cloudy and hard to see through. thanks shawn jackson Harvard Univ Boston, MA
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