When I needed a quick human PBMC sample to check whether an antibody had gone off or something I would layer 0.5ml diluted blood onto 0.5ml ficol in a 1.5ml microcentrifuge tube. I'd then spin it at 3000rpm for 5 minutes. I never optimized it for yield as I didn't care if I lost some cells, seemed to work quite well though. Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352
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