You can get the L428 at : http://www.dsmz.de/ Best regards, Joost Schuitemaker _________________________________________ J.H.N. Schuitemaker Research Technician Dept. Cell Biology & Histology Cellular Immunology Group P.O.box 22700 1100 DE Amsterdam The Netherlands Tel. +31 (0)20 5664960 Fax. +31 (0)20 8725456 E-mail privé: J.H.N.Schuitemaker@consunet.nl -----Original Message----- From: Kudinov, Yuri [mailto:YKudinov@CHW.edu] Sent: dinsdag 14 maart 2000 6:32 To: cyto-inbox Subject: Human dendritic cell lines? Does anyone know a commercial source of human dendritic cell lines? Or just any source? I could find only one article on this subject. See Nunez R. et al. "Characterization of two human dendritic cell-lines that express CD1a, take-up, process and present soluble antigens and induce MLR" Immunol.Lett.1998 Mar;61(1):33-43. http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9562373&form=6 I have learned recently that cell lines isolated from patients with histiocytosis and Hodgkin's disease often show features of mature DC's. For example, a Hodgkin's disease cell line L428 has potent APC activity and a phenotype which includes MHC Class I/II(high), CD80/86(+), CD83(+) and CD40(+). I searched ATCC online catalog and it appears they don't have it. Any ideas where I can get that cell line (L428)? It must be very popular (77 Medline references). I've also learned that acute myeloid leukemia cells can be induced to differentiate into dendritic cells by treatment with interleukine mixtures, see http://www.bloodjournal.org/cgi/content/full/93/3/780 Can anyone recommend me an AML cell line, or a monocytic/histiocytic cell line? An ideal candidate would be capable of differentiating into something CD80/86(+) ... and IL12(+) ... and it would be just great if it also happens to be HLA-A2(+). Any help would be greatly appreciated. Thank you, Yuri Kudinov, postdoctoral fellow SVMC, Immunotherapy lab 201 S.Alvarado St, Ste 312 Los Angeles, CA 90057-2354
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