Can anyone recommend a good antibody against mouse IgG to be used as a primary antibody on murine B cells? So for I've tried FITC conjugated polyclonals against IgG and IgG1 from Southern Biotech and Sigma respectively and home grown mMb 20.9 (anti-IgG1 a allotype). The polyclonals seem to cross react with sIgM/D. The monoclonal while working well in ELISA failed to stain at all by FACS. The polyclonals also cross reacted strongly with rat mAbs which is a problem as I want to stain for multiple surface markers. Any suggestions much appreciated. Jennifer ______________ jen.bell@ed.ac.uk Institute of Cell, Animal and Population Biology University of Edinburgh Kings Buildings Edinburgh EH9 3JT UK tel. 0131 650 5556 fax. 0131 650 7322
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:12 EST