re: BD LSR ?

From: Howard Shapiro (hms@shapirolab.com)
Date: Sat Mar 04 2000 - 17:04:15 EST


Howard Petrie writes:



>We have had our instrument [B-D LSR] for about 3-4 months, and at times
>(see below)
>the data have been incredibly good, with DNA cv's of 3-5% and no
>discernable noise (HeCd lasers must have improved significantly).

So far, so good, but the noise problem with a He-Cd laser typically arises
after months of operation.  I have pretty consistently gotten DNA CV's
under 2% (assuming the cells are that good) with new He-Cd lasers, even
those made ten and more years ago.  For what it's worth, my newest He-Cd is
pretty much the same model I am told is used in the LSR, and, while it has
gotten somewhat noisier over its time in operation, it is better than the
older ones.  Running a He-Cd a few hours a week is the best way to slow
down the development of the noise problem, and it sounds as if that will
happen automatically in Howard Petrie's lab.  The prognosis is good.

-Howard Shapiro



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