1) Does anyone have experience evaluating degranulation of eosinophils (or other granulocytes) by flow cytometric analysis? (Using either scatter or intracellular stain) 2) I received a lot of queries regarding a previous question, but no "answers". Does anyone have any ideas? " I am measuring cell surface receptors for cytokines. How do I know if a decrease in fluorescence is due to modulation of receptor or to masking of the receptor by cytokines that are acquired in vivo? I have tried, unsuccessfully, to detect surface-bound cytokine before and after incubating cells with cytokine. Is there a way to dissociate ligand-receptor complexes without altering the receptor in the process?" Thanks-Becky
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