It is unclear to me just what you are using 7-AAD for, much less the vague
"PE." During the recent discussion about the difficulties of using CFSE on
human lymphoid cells, I already described using 7-AAD to discriminate live
from dead cells. I encountered no problems in separating the emission of 7-AAD
from that of CFSE. People also described how to discern CFSE from PE
emissions. I would suggest that you go into the Cytometry e-mail archives and
search for "CFSE".
Kevin G. Waddick, Ph.D.
Parker Hughes Institute
Kate Dugan wrote:
> To Whom This May Concern,
>
> My name is Kate Dugan and I am a graduate student in Dr. Tim Manser's lab
> at Thomas Jefferson University. I want to do a cell cycle analysis using
> CFSE, PE, and 7-AAD. I tried this one time but without success because
> of the 7-AAD staining. I was wondering if you had any suggestions for using
> 7-AAD in this application. I will greatly appreciate any information that
> you could share with me. Thank you for your time.
>
> Sincerely,
>
> Kate Dugan
> ***********************************************
> Kate Dugan
> Thomas Jefferson University
> BLSB 708
> 233 South 10th Street
> Philadelphia, PA 19107
> Lab: 215-503-4545
> Fax: 215-923-4153
> Email: K_Dugan@lac.jci.tju.edu
> ***********************************************
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