Be carefull in differentiation of cells by AS-02. This mAb recognizes a Thy-1 antigen (as published by Saalbach,A. et al recently) not only on fibroblasts but also activated endothelial cells. Ulrich Sack Joost Schuitemaker schrieb: > > You can label your fibroblasts with a specific Ab; ASO-2 (mouse IgG1). As > far as we know only sold by Dianova, PO box 301250, D-20305 Hamburg, > Germany. Tel +49 40 45 0607. fax +49 40 45 06 7490. Litt. J of Inv Derm. > Vol106, no 6, june 1996, 1314 -1319. > Keratinocytes is more difficult. > > Success, > > Joost Schuitemaker > > _________________________________________ > > J.H.N. Schuitemaker > Research Technician > Dept. Cell Biology & Histology > Cellular Immunology Group > P.O.box 22700 > 1100 DE Amsterdam > The Netherlands > > Tel. +31 (0)20 5664960 > Fax. +31 (0)20 8725456 > > E-mail privé: J.H.N.Schuitemaker@consunet.nl > > -----Original Message----- > From: Glennelle Washington [mailto:gcw@helix.nih.gov] > Sent: vrijdag 25 februari 2000 23:38 > To: cyto-inbox > Subject: Differentiation of Fibroblasts and Keratinocytes > > A colleague inquired as to what reagents should be used to differentiate > fibroblasts and keratinocytes in culture. How would one label these cells > for sorting? Please send responses directly to henry.rodriguez@nist.gov > > Thank you in advance for your responses.
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:09 EST