We take a sample put in RPMI and push & rub gently through wire mesh with the plunger of a glass syringe, using more RPMI as lubricant. In order to lose as few cells as possible, we will take the cell suspension we have created, and put it in as many tubes as we will need for out panel, spin the tubes down, aspirate off the supernatant & add the antibody. If it is in fact a lymphoma, there are usually sufficient intact cells. We have never tried aspirating the lesion. kathy altig -----Original Message----- From: owner-cytometry@flowcyt.cyto.purdue.edu [SMTP:owner-cytometry@flowcyt.cyto.purdue.edu] Sent: Thursday, February 24, 2000 7:10 AM To: Cytometry Mailing List Subject: Has any one gotten good cell recovery with surface antigens intact from skin biopsies? The cells are adult T cell leukemia/lymphoma cells and are a little fragile. We tried mincing and just got debris. We are thinking of aspirating the lesion. Any bright ideas? Maryalice Maryalice Stetler-Stevenson Director Flow Cytometry Unit Laboratory of Pathology, NCI, NIH
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