We did quantitate Ca++ using indo-1 and a FACS 440- but the instrument used shouldn't matter, as long as you excite at 355-357, monitor 405/485 with a ratio board or digital data so you get the ratio for each cell. I'll try to find the reference where we published this and e mail it to you. Elizabeth R. Simons, Ph.D. Professor of Biochemistry Boston University School of Medicine 80 E. Concord Street Boston, MA 02118 phone 617-638-4332 FAX 617-638-5339 e mail esimons@bu.edu On Fri, 25 Feb 2000 Akos_Szilvasi@biogen.com wrote: > > > > Hi, Flowers, > > We would like to quantitate intracellular Ca concentration using Indo-1 > on the > MoFlo. Does anyone have experience with the quantitation procedure? Would > you > share it with me? > > Thanks, Akos > > > Akos Szilvasi > Biogen > > >
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