Meeting Announcement Flow Cytometry Workshop: Principles and Applications Tuesday, March 14, 2000 9 am - 5 pm US EPA-ERC Auditorium 86 Alexander Drive Research Triangle Park, NC Located on the corner of Alexander Drive and NC Highway 54 (red brick building). Use the Alexander Drive entrance. Program schedule: 9:00 - 9:50 Mark Edinger (Technical Applications Specialist, BD Biosciences, San Diego, CA) "An Overview of Flow Cytometry" 9:50 -10:00 Robert Zucker (US EPA, Research Triangle Park, NC) "The Relationship between Fluidics and Lasers" 10:00 -10:15 Break with refreshments 10:15 -11:05 Tim Kute (Wake Forest University, Winston Salem, NC) "DNA Analysis" 11:05 -11:15 Robert Zucker (US EPA, Research Triangle Park, NC) "Some Factors Effecting DNA Analysis" 11:15 -12:00 Roundtable discussion of QA issues and flow cytometer maintenance. Participants include: Darren Hickerson (East Carolina University, Greenville, NC), Mike Cook (Duke University, Durham, NC), Robert Zucker (USEPA, RTP, NC), Scottie Sparks (University of North Carolina, Chapel Hill, NC), Tim Kute (Wake Forest University, Winston Salem, NC) and Marie Iannone (Glaxo Wellcome, RTP, NC) 12:00 -1:00 Lunch (pizza or US EPA Cafeteria) 1:00 - 2:00 Mark Edinger (Technical Applications Specialist, BD Biosciences, San Diego, CA) "Flow Cytometry Applications: Old and New" 2:00 - 2:30 Darren Hickerson (East Carolina University, Greenville, NC) "Flow Cytometry Core Stories and Ca2+ Influx" 2:30 - 2:45 Break with refreshments 2:45 - 3:30 Art Bode (East Carolina University, Greenville, NC) "Detection of Small Particles: Size Matters" 3: 30 - 4:00 Marie Iannone (Glaxo Wellcome, Research Triangle Park, NC) "Multiplexed Analysis using Fluorescent Microspheres" 4:00 - 4:15 Robert Zucker (US EPA, Research Triangle Park, NC) "Apoptosis: Factors Effecting Flow Data and Artifacts in the Cytometry Literature" 4:15 - 5:00 Continuation of roundtable discussion of QA issues and flow cytometer maintenance. Results of the FCSC Instrument Performance Survey will be available. We gratefully acknowledge BD Biosciences for reimbursing the expenses of this meeting. For further information about this meeting contact Robert Zucker at (919) 541-1585 or zucker.robert@epa.gov <mailto:zucker.robert@epa.gov>. This meeting is offered without charge due to the generous support of BD Biosciences. Seating may be limited. Please register early (by emailing Robert Zucker at zucker.robert@epa.gov <mailto:zucker.robert@epa.gov> or calling 541-1585) so the organizers can anticipate the number people for materials and refreshments. Lunch (pizza) will be provided to registered attendees. A cafeteria is also available. This should be an enjoyable workshop in flow land. Bring your questions and open your minds. Knowledge will flow. Cheers, Sam Sam Witherspoon sw11527@glaxowellcome.com Dept. of Receptor Biochemistry Tel. 919-483-3078 Glaxo Wellcome R&D Page 919-857-7768 5 Moore Dr. Fax 919-483-0585 RTP, NC 27709
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