you need to remember that the CD34 cells are considered stem cells and from the data available for Rhodamine 123 and Hoechst staining of these cells, they exhibit a highly active MDR system. It is possible that the cells are pumping the indo out as fast as it is going in. Charles A. Kuszynski, Ph.D. Assistant Professor/Director University of Nebraska Medical Center 986495 Nebraska Medical Center Cell Analysis Facility, WH 3024 Omaha, NE 68198-6495 402.559.6267 voice 402.559.4077 fax ckuszyns@unmc.edu
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