I would like to know if anyone has any experience loading CD34 cells with Indo-1. We have tried and seem to be getting a good proportion that are not loading while others seem to load. We have tried several concentrations and the result we are getting does not seem to be related to dye concentration. Any suggestions? David M. Dombkowski dombkowski@helix.mgh.harvard.edu Flow Cytometry-Pathology-CNY rm7017 149 13th Street Massachusetts General Hospital-East Boston, MA 02129 Tel. (617)726-1683 Fax.(617)724-3164
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