Indo-1 and cd34 cells

From: David Dombkowski (helix.mgh.harvard.edu@helix.mgh.harvard.edu)
Date: Thu Feb 24 2000 - 18:04:46 EST

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 I would like to know if anyone has any experience loading CD34 cells with
Indo-1. We have tried and seem to be getting a good proportion that
are not
loading while others seem to load. We have tried several concentrations
and
the result we are getting does not seem to be related to dye
concentration.
Any suggestions?

David M. Dombkowski
dombkowski@helix.mgh.harvard.edu
Flow Cytometry-Pathology-CNY rm7017
149 13th Street
Massachusetts General Hospital-East
Boston, MA 02129
Tel. (617)726-1683
Fax.(617)724-3164

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