Streptav. Second makes signal brighter?

From: Jeff Haug (jhaug@im.wustl.edu)
Date: Thu Feb 24 2000 - 18:40:35 EST 

I have always been told that a biotin conjugated primary antibody followed
by a fluorescent conjugated streptavidin secondary antibody is a method
that should make a low density signal more bright.

Is this really true or just theoretical?

I ask this question because when I have performed comparisons between
direct and indirect staining methods I have never seen much difference.

Thanks, Jeffrey

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