Dear Gayle, I have labelled cell lines (K562 and Daudi) with calcein-AM with no problem. The cells were incubated with 50 nM calcein-AM for 15 minutes at 37 degrees in serum-free medium, then washed with medium + 10% FCS. The fluorescence was measured on an ELITE, collected through a 525 nm BP filter. The calcein fluorescence was bright but could be compensated out of the PMT used for detecting PE (the PE fluorescence passed through a 550DL filter then a 575BP filter). Malcolm King Dept Clinical Immunology Royal North Shore Hospital St. Leonards NSW 2065 Australia. >>> Gayle Hatleberg <GayleHatleberg@probes.com> 02/17/00 04:10am >>> Hi all, I am wondering if anyone has ever used Calcein Am in flowcytometry. I am having problems compensating it. I would love your advice. Gayle Hatleberg Molecular Probes, Inc.
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