Don't the CD4 sometimes fall off under activation? Thus you might need a few more markers. Eleven Colours might do. Regards Gerhard -----Original Message----- From: Calman Prussin [SMTP:CPRUSSIN@niaid.nih.gov] Sent: Tuesday, February 15, 2000 9:59 PM To: Cytometry Mailing List Subject: RE: Wich cells are il-4 producers???? Actually, the NK T cells that are outrageous IL-4 expressers are CD4+. See the early papers by authors Paul and/or Bendelac in mouse and our paper in humans. > ---------- > From: Samuel Lundin > Sent: Tuesday, February 15, 2000 4:35 AM > To: Cytometry Mailing List > Subject: Re: Wich cells are il-4 producers???? > > > At 09:14 2000-02-11 -0200, Zilton Farias Meira de Vasconcelos wrote: > > > >Dears, > > > >Anybody knows about cells in the lymnphoid-gate that are CD4- and CD8- > and > >IL-4+ > >This is spleen cells of a balb/c with leishmania???? > > > >And the cytometry reveals these cells???? > > > >Thank's > > > > They might be NK-T cells, I presume. A large part of the T cells with NK > receptors are CD4 and CD8 negative, and prone to IL-4 production. > Have you checked that? > > Best regards, > > ============================================ > Samuel Lundin, PhD > Dept. of Medical Microbiology and Immunology, > Göteborg University > Guldhedsgatan 10A, 413 46 Göteborg, Sweden > Phone: +46-31- 826661 / 3424688 > Fax: +46-31-826976 >
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