Hi Deb, I lived, but it wasn't pretty. I once tried using Sepharose as a model for solid phase binding studies, but repeatedly clogged the 'sipper' of a FACSort and was glad when I finally got the tube cleared. I cannot remember the swollen size of the particles, but at the time was assuming (wrong !!) that they were significantly smaller than the I.D. of the sipper. I would recommend that your colleague procure some smaller coated particles which should be available from places like Spherotech, (www.spherotech.com), unless he can guarantee that his beads are 30-40 microns or less in diameter. > -----Original Message----- > From: Deborah Berglund [SMTP:umbbd@gemini.oscs.montana.edu] > Sent: Tuesday, February 15, 2000 3:41 PM > To: Cytometry Mailing List > Subject: separose beads in a facscan > > > > Hi everybody, > > A colleague wants to know if anyone has looked at sepharose beads in a > FACScan and lived to tell about it. He wants to bind a fluorescent > protein to an Ab, then bind the whole works to protein A coated sepharose > bead, then analyse the relative fluorescence with our FACScan without > gacking up the tubing, etc. > > Thanks, > Deb Berglund > Montana State University >
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