Hi Everybody: We're trying to measure Ki-67 expression in naive and memory T cells from frozen PBMC samples. I've heard anecdotes that results from fresh and frozen samples are similar; however, I don't seem to find this. I was wondering if anyone has experience measuring Ki-67 in frozen cells and what techniques, tips, or tricks they might have to keep results similar to those of fresh cells. I have tried using both the Immunotech Fix and Perm and the Ortho Permeafix kits. I've also tried using Ki-67 antibody purchased from Immunotech and from Pharmingen. I don't think the viability of the thawed cells is in question. I'd appreciate any advice you all have... Thanks, Pratip... ______________________________________________ Pratip Chattopadhyay pchattop@jhsph.edu (410) 955-7205
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