Deneen, I've not used these cells, but from what I've read you shouldn't have any problems reading these as any other fibroblast-like cell. They may be relatively large, so there may be an advantage to using log FSC/log SSC. This will allow you to compress the signal into a better manageable cluster. Keep in mind that these types of cultures are more likely to clump, and may grow as clusters that are more tricky to render as single cells. Viral infection leading to growth perturbations and expanded cell death will add to these complications. Your best ally in developing this assay is the microscope . . . microscopic viewing can often eliminate some of the mystery of flow cytometry. "Kozoriz, Deneen HE0" wrote: > Hello Fellow Flowers > > Our lab has been using the BD FACSCalibur for Leukemia/Lymphoma and CD4/8 > testing. Our clinical director wants us to expand the Flow Cytometry > section and develop procedures for anti-viral susceptibility testing. Our > first project is anti-viral drugs for HSV using Vero cells. > We have encountered a few stumbling blocks (i.e.: what do Vero cells look > like FSC vs SSC???/ should the graphs be Log or Lin etc.). > Is anyone working in this area? I would really like to pick your brain. > Any response would be greatly appreciated. > > Thanking you in advance > > P.S. Happy Valentine's Day > > Deneen E. Kozoriz B.Sc. > Provincial Laboratories > Regina, Sask. Canada > > ------------------------------------------------------------------------ > > Part 1.2 Type: application/ms-tnef > Encoding: base64 -- Mark A. KuKuruga, Managing Director University of Michigan Core Flow Cytometry <http://www.cancer.med.umich.edu/flow_cytometry> phone: 734-647-3216 fax: 734-936-7376 kukuru@umich.edu
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:07 EST