Anti-Viral Susceptibility Testing using Flow

From: Kozoriz, Deneen HE0 (dkozoriz@health.gov.sk.ca)
Date: Mon Feb 14 2000 - 11:32:32 EST


Hello Fellow Flowers

Our lab has been using the BD FACSCalibur for Leukemia/Lymphoma and CD4/8
testing.  Our clinical director wants us to expand the Flow Cytometry
section and develop procedures for anti-viral susceptibility testing.  Our
first project is anti-viral drugs for HSV using Vero cells.
We have encountered a few stumbling blocks (i.e.: what do Vero cells look
like FSC vs SSC???/ should the graphs be Log or Lin etc.).
Is anyone working in this area?  I would really like to pick your brain.
Any response would be greatly appreciated.

Thanking you in advance

P.S. Happy Valentine's Day

Deneen E. Kozoriz B.Sc.
Provincial Laboratories
Regina, Sask. Canada





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