ploidy analysis of Arabidopsis leaf cells

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Hello,

I'd like to compare the ploidies of Arabidopsis leaf cells between wild
type and a mutant.  Does anybody know a good protocol of flow cytometry
for this analysis (including the sample preparation, dye, et al.)?
The tissues would be rossette leaves.

Please respond to my email address since I'm not on the cytometry mailing list.  Thank you very much for your time and help.

Jian  Hua

Jian Hua
Whitehead Institute
9 Cambridge Center
Cambridge, MA 02142
email: jhua@wi.mit.edu

• Next message: martin gerard kelly: "Re: Annexin V (AV) and propidium iodide (PI) staining to identify apoptotic/necrotic cells"
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