one crazy sample - "big cells"

From: Maciej Simm (simmmmer@yahoo.com)
Date: Wed Feb 09 2000 - 19:34:13 EST


Greetings, 

We received a specimen which gave me a headache. 

The patient is a preterm neonate.

The leukogate tube has shown a ball of cells which are
brighter than grans in CD14 and are also higher in SSC
than grans. (http://www.cd4cd8.com/page1.html). 

The burst test has further shown that the big granulocytes
are highly phagocytic without any stimulation.
(http://www.cd4cd8.com/page2.html)

Finally the burst test yielded two more strange populations
in the scatter plot after incubation of whole blood with 
opsonized ecoli. (http://www.cd4cd8.com/page3.html), all of
which are phagocytic (note that normally only 2 populations would
be phagocytic : monocytes and granulocytes. this plot shows that
there are monocytes (ungated) small/big grans (R2) and two 
weird populations that I don't know where they came from (R3 and
R4 for a grandiose total of 5 phagocytic populations). 

If anyone has seen anything like that before or would like to
speculate please let me know. Again I plan to post a summary 
of answers on the website as well as here. 

Thanks for your time, 

Maciej

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