At 10:00 AM -0800 1/28/00, Barsky, Lora wrote: >Does anyone recommend the BD Omnicomp (interbeam compensation unit) for the >FACS Vantage? I'm in the position to acquire more equipment for our Vantage >and could use some recommendations, yay or nay. > >I would imagine it would be absolutely necessary when using the new tandem >dyes that are becoming available (PerCP-Cy5.5 = TruRed, APC-Cy7 = PharRed >or PE-Cy5. > >What do you think? > >-Lora Barsky >CHLA >Researach Immunology/BMT Just be aware that Omnicomp only compensates between laser 1 to laser 2 and not in the opposite direction (despite the "omni" name). So you cannot compensate APC and PE-CY5 in both directions and this is actually a problem. I don't think Omnicomp will help you when using Phar Red, but will be necessary for using PE-CY5 to get the signal out of the second laser; probably same for Tru-Red. Nonetheless, as mentioned, it will not help get the APC signal out of the laser 1 system and APC is in fact excited a bit by the first laser at 488. Mark Shlomchik, MD, PhD Associate Professor of Laboratory Medicine and Immunobiology Yale University School of Medicine 203-688-2089 203-688-2748 (fax) mark.shlomchik@yale.edu
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