Geoff, You might want to try using Terasaki plates instead of 96well plates, the wells are easier to scan for the presence of single cells. Also it might be worth giving the 96well plates (as well as the Terasaki plates) a brief spin after the cell deposition, to make sure all cells are actually at the bottom of the well. I have very good experience with the single deposition on the Vantage (>96%). Nicole Nicole Baumgarth Stanford University School of Medicine Dept Genetics 300 Pasteur Drive, Beckman Center B007 Stanford, California 94305 - 5318 FAX: 650 - 725 8564 Phone: 650 - 723 7149
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