Hello Everyone, Occasionally, I have to thaw some PBMC's for Flow. They are frozen in DMSO in 1 mL aliquots. The thawing procedure is as described on the instruction sheet that comes with the DMSO. I am wondering what kind of yeilds people get when thawing PBMC's. Is it normal to recover only 50% of the cells? These are rhesus PBMC's and are infected with HIV/SIV/SHIV. I would appreciate any information anyone could share. Thank you very much Dagna Sheerar Wisconsin Regional Primate Research Center University of Wisconsin - Madison 1220 Capitol Court Building #2 Room #215 Madison, WI 53715 608 265 3796 dagnas@primate.wisc.edu
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:04 EST