Michael Ormerod wrote: > > I had a request for help from Yanhui Yang in China in analysing DNA histograms. > > I suggested using WINMDI to create DNA histograms and then Cylchred to analyse them > (see below). However, he was unable to read his data files using WINMDI. > > I have never had a problem reading BD files supplied to me by other people. > I do not have direct access to a Cellquest system to explore this directly. > Can anyone help? > > Thanks, > > Michael Ormerod > > -------------Forwarded Message----------------- > > From: Yanhui YANG, INTERNET:yhyang@jzw.cern.ac.cn > To: Michael Ormerod, Michael_Ormerod > > Date: 17/01/00 23:09 PM > > RE: Re: help > > > Dear Dr.Ormerod, > > Thank you very much for your kindness and the information provided. I've downloaded > those free softwares and tried my data on them. But unfortunately, there was > something wrong when the downloaded software WinMDI reading my data files. It was > said that the files were not FCS files. Actually, the data were originally acquired > in Japan by a FACSCalibur FCM with a software of CellQuest running on Mac, and then > transfered into PC readable format for the reason that we do not have a Mac here. I > wonder if it is this transfermation cause the error in reading the files? If so, how > can I make the analyze feasible? I'll be very grateful if you would provide further > help. > > Thanks again and sorry for the bother. > > Best wishes > > Sincerely, > Yanhui > > In 00-1-17 17:00:00 you wrote: > >If you have your data in a listed data file in FCS format (used by all > >modern flow cytometry systems) you can produce histograms in FCS format > >using WINMDI. > > > >You can then analyse these files using Cylchred. > > > >Both these programs are available free on the Internet. > > > >WINMDI is available from http://facs.scripps.edu. > > > >Cylchred is available from http://www.uwcm.ac.uk/uwcm/hg/hoy/software.html. Mike: I run CQ and have used the data files in WinMDI. From the Mac, I'll copy the file(s) to an IBM disk and then take the disk over to the IBM with WinMDI. For reasons already discussed, the transfer is uni-directional (for all practical purposes). Once saved to an IBM disk, the copied file can not be re-opened in CQ unless certain things are done. To the point: Occasionally, say 10-15% of the time, a file saved from the Mac on an IBM disk will not open with WinMDI while other files on the same disk open just fine. I "write it off" as just another nuisance of saving and transporting data between platforms. I find that re-copying the file to another disk solves the problem most of the time. I have had rare exceptions and have had to copy the file a third time. Another solution was that we do an initial archive to a unix server from the Mac. There, both platforms can read the data with CQ on the Mac and WinMDI on the IBM. Final archiving to CD-R is performed only with the Mac, in this way, data can still be read by both applications. I hope this helps, David =========== David L. Haviland, Ph.D., Asst. Prof. Immunology University of Texas - Houston, H.S.C. Institute of Molecular Medicine, R907 2121 W. Holcombe Blvd., Houston, TX 77030 713.500.2413-Voice//713.500.2424-FAX ----------------- If everything seems to be going so well, you have obviously overlooked something. ==========
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