Dear Diane, I have done this with some limited success with budding yeast. I used a methods provided by my client from Methods in Enzymology, Vol 283, pp 322 - 332, 1997, Academic Press. [24] "Flow Cytometric Analysis of DNA Content in Budding Yeast" by Steven B. Haase & Daniel J. Lew There is not a great deal of difference from mammalian cells. Regards Rob W. At 11:41 01/18/2000 -0500, you wrote: >Hello all, > >I have recently been asked to run cell cycle on yeast. I have run endless >samples for cell cycle on tumor lines, but never on yeast. I will be >using propidium iodide. Is this a difficult assay? I would appreciate >any comments from folks who have already run this type of sample. >Thanks in advance for your help! > >Diane Sharp >DuPont Pharmaceuticals >Glenolden, PA > R. Wadley, B.App.Sc. M.L.S, Grad.Dip.Sc.MM Laboratory Manager Cellular Analysis Facility School of Microbiology & Immunology UNSW, New South Wales, Australia, 2052 Ph (BH) +61 (2) 9385 3517 Ph (AH) +61 (2) 9555 1239 Fax +61 (2) 9385 1591 E-mail r.wadley@unsw.edu.au www http://www.micro.unsw.edu.au/caf.html
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