I have run samples on FacsCalibur with this combination and it works quite well. If using 7AAD for viability there is very little problem setting compensation with the most adjustment necessary between 7AAD and PE. If using 7AAD for DNA cell cycle this adjustment is a little more involved as the brightness of 7AAD requires carefully compensating with PE. I have seen no problem with 7AAD in the HE-NE APC signal. David Dombkowski MGH-Pathology-Flow Cytometry David M. Dombkowski dombkowski@helix.mgh.harvard.edu Flow Cytometry-Pathology-CNY rm7017 149 13th Street Massachusetts General Hospital-East Boston, MA 02129 Tel. (617)726-1683 Fax.(617)724-3164
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