Thanks for all your responses.
Here's a summary:
reagents people use (no special order here) (exerpts copied and pasted)
1. UIC2 (PE) of Immunotech (Cat. N IM2370) and BD PGP (Cat N 340555)
2. "Recently Immunoquality producs develop a line of products of drug
resistence and include antibodies to MDR, LRP and MRP."
3. Kamiya brand: "We have found MRK16 to give the highest level of
fluorescence of all the commercially available anti-P-glycoprotein
mAbs that we have tried."
4. "Use the 4E3 antibody from Signet it stains a similar epitope as MRK-16,
they are both external epitopes and very reliable the cat# is 8745-01
and comes already conjugated to FITC. The company also sells a negative
control antibody also conjugated to FITC Cat # 8746-01. 4E3 does not cross
react with MDR3 gene product"
5. "we have used the BD stain - it does NOT work. Neither does the Coulter
one. "
References people recommended:
Kunikane H. Zalupski MM. Ramachandran C. KuKuruga MA. Lucas D. Ryan JR.
Krishan A. Flow cytometric analysis of P-glycoprotein expression and drug
efflux in human soft tissue and bone sarcomas. Cytometry. 30(4):197-203,
1997 Aug 15
Reference I found interesting:
Sadhir Gupta, Sudeepta Aggarwal P-Glycoprotein in Cells of the Human
immune system. The immunologist, May/June '96, Vol. 4 No.3
=====
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| Maciej S. Simm | 525 E 68th Street |
| Research Technician | Room N-805 |
| Cornell Medical Center | Tel. 212.746.3428 |
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