Dear Neo Sim Yee, In our work with bacteria we always make a final adjustment of the optics using small (1 micron) beads. The problem in using "big" beads is that you can consider optics adjustment is fine, but you do not control the background noise that can be the "tricky" part for detecting small bacteria. The relationship between signal and noise should be as high as possible, and you can hardly control noise levels with the 10 microns beads. Hope it helps, Jaume En/Na Neo Sim Yee ha escrit: > Dear all, > > I have an enquiry with regards to the flow check paticles that are used for > assessing the optical alignment of the flow cytometer. > > In our work, we are dealing with bacteria of a size range as small as 0.5um. > The flow check we are currently using is the 10um Flow Check Fluorosphere > from Coulter. Noting the relatively large difference in size of the flow > check particles and the cells that we are analysing, does this introduce a > significant amount of error in the sense that the instrument may not be > optimally aligned fot the analysis of such small paticles? > > Thank you for your help. > > Kind Regards > > Neo Sim Yee, Lezlie > Department of Civil Engineering > National University of Singapore -- Jaume Comas Citometria de Flux. Serveis CientíficoTècnics Universitat de Barcelona tel 34/93.402.13.46 fax 34/93.402.13.98 mailto:jaume@giga.sct.ub.es http://giga.sct.ub.es/personal/fitxes/jcr460.htm
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