Re: Sorting and room temperature

From: Arnold Pizzey (a.pizzey@ucl.ac.uk)
Date: Tue Jan 11 2000 - 06:15:18 EST


>Hi Everyone,
>
>I seem to remember a number of  years ago, that the room temerature
>adversely affected the FACScan's ability to operate....I haven't noticed
>such an affect with the FACsort I now use...perhaps the heat fluctuations we
>experienced while our bldg was being renovated didn't affect *that*
>particular room as much...at least when *I* was using the FACSort.
>
>Today it was 83 degrees F. (and probably warmer as my Vantage is in a back
>corner of the room) and I had a horrible time trying to get stabilized
>streams or a reasonable drop delay. Was it me or the  room temp?
>
>Thanks!
>
>Tricia
>

Hello Tricia

This thread has been covered in some detail previously (worth checking the
archive), to summarise however, I (and I believe many others)  found that a
constant (low) temperature is required to guarantee success in sorting.

Arnold


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	Arnold Richard Pizzey
	Department of Haematology
	Royal Free and University College London Medical School
	98 Chenies Mews
	London WC1E 6HX
	U.K

	voice:	+44 171-209-6234
	Fax:	+44 171-209-6222
	email:	a.pizzey@ucl.ac.uk
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